Time-dose response of Trypanosoma congolense bloodstream forms to diminazene and isometamidium
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Date
1994Author
Kaminsky, R.
Chuma, F.
Wasike, R.P.N.
Type
ArticleLanguage
enMetadata
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Trypanosoma congolense bloodstream forms were propagated in vitro axenically in a
simplified cultivation medium at 34 ° C. Viability of a drug-sensitive and a drug-resistant
clone were examined for 10 days following exposure to 0.1, 1.0 and 10.0/~g ml- 1 of diminazene
aceturate and 0.1, 1.0 and 10.0 ng ml-~ of isometamidium chloride for various
time intervals. Drug-sensitive T. congolense were irreversibly damaged after incubation
with 10/~g ml- ~ or 1 #g ml- 1 diminazene aceturate for 30 min or 2 h, respectively, while
drug-resistant trypanosomes were not affected. Exposure to 10 ng ml-~ isometamidium
chloride eliminated drug-sensitive trypanosomes after 24 h and drug-resistant trypanosomes
after 96 h. The data obtained on in vitro time-dose responses of T. congolense were
related to pharmacokinetic data of diminazene and isometamidium in cattle plasma.
Citation
Veterinary Parasitology 52 (1994) 235-242Publisher
International Laboratory for Research on Animal Diseases (ILRAD)