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dc.contributor.authorApopa, P.L
dc.contributor.authorMajiwa, A.O
dc.contributor.authorKinyanjui, P.W
dc.date.accessioned2013-03-21T09:36:56Z
dc.date.issued2004
dc.identifier.citationEast Africa Journal of Life Sciences 5(1):37-48(2004)en
dc.identifier.urihttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/14882
dc.description.abstractGenomic DNA from T. congolense clone I~1180 was purified and resolved by pulsed field gradient gel electrophoresis (PFGE) technique to separate the genome into chromosome-sized DNA molecules. PFGE conditions most ideal for separation of the four medium-sized chromosomes were optimized. The sizes of the four medium-sized chromosomes were determined as 340 Kb, 360 Kb, 400 Kb and 500 Kb (designated chromosomes 1-4). The separated chromosome bands were individually cut from agarose gel and DNA purified. Each chromosome DNA sample was PCR-amplified with short oligonucleotide primers (10 mer) of arbitrary nucleotide sequences. The fingerprints of DNA fragments generated with these primers were resolved by standard agarose gel electrophoresis. The majority of the random primers used generated reproducible fingerprints, some of which were polymorphic for the different chromosomes amplified. A DNA fragment, which hybridizes to only one of the four medium-sized chromosomes, was identified.en
dc.language.isoenen
dc.subjectPFGEen
dc.subjectTrypanosome chromosomeen
dc.subjectPCRen
dc.subjectDNA polymorphismen
dc.titleIdentification of DNA markers specific to medium-sized chromosomes 4 of Trypanosoma congolenseen
dc.typeArticleen
local.embargo.terms6 monthsen
local.embargo.lift2013-09-17T09:36:56Z
local.publisherIntemational Livestock Research Instituteen
local.publisherDepartment of Biochemistry, University of Nairobien


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