Exovesicles from human activated dendritic cells (DCS) fuse with resting DCS allowing them to present allo-antigens
Date
2006-12Author
Obregon, C
Rothen-Rutishauser, B
Gitahi, S K
Gehr, P
Nicod, L P
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Dendritic cells (DCs) can release microvesicles, but the latter's numbers, size, and fate are unclear. Fluorescently labeled DCs were visualized by laser-scanning microscopy. Using a Surpass algorithm, we were able to identify and quantify per cell several hundred microvesicles released from the surface of stimulated DCs. We show that most of these microvesicles are not of endocytic origin but result from budding of the plasma membrane, hence their name, exovesicle. Using a double vital staining, we show that exovesicles isolated from activated DCs can fuse with the membrane of resting DCs, thereby allowing them to present alloantigens to lymphocytes. We concluded that, within a few hours from their release, exovesicles may amplify local or distant adaptive immunological response
URI
http://www.ncbi.nlm.nih.gov/pubmed/17148675http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/16573
Citation
American Journal of Pathology. 2006;169(6): 2127-36Publisher
Wangari Mathai Institute, University of Nairobi
Description
Journal article