Substantial Intrapatient Differences In The Breadth And Specificity Of Hiv-specific Cd8+ T-cell Interferon-gamma And Proliferation Responses.
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Date
2008-10Author
McKinnon, LR
Ball, TB
Wachihi, C,
Chinga, N
Maingi, A
Luo, M
Fowke, K R
Plummer, FA
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
HIV vaccine design and evaluation require a better understanding of protective immune responses. HIV-specific CD8+ T-cell responses have been characterized extensively using interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot (ELISPOT) assays, which do not always correlate with control of viral replication or disease progression. Alternative aspects of CD8+ T-cell responses, in particular those associated with a central memory (Tcm) phenotype, may be more protective against disease progression. To determine the extent that the breadth and specificity of HIV-specific CD8+ T-cell responses differ based on immunological readout, we screened in HIV-infected Kenyan sex workers for responses to HIV Env using IFN-gamma ELISPOT and 6-day carboxyfluorescein succinimidyl ester-based proliferation assays. This comparison revealed substantial differences in the epitopes recognized when the assay readout was IFN-gamma versus proliferation. Although 24 and 41 IFN-gamma and proliferative responses were identified, overlapping specificity was observed for only 5 responses. Breadth also differed between assays in several patients. Env-specific IFN-gamma breadth was found to correlate inversely with CD4 count (r = -0.66, P = 0.005), although this was not the case for proliferation. These data suggest that efforts to define HIV-specific CD8+ T-cell responses may need to be revisited using additional immunological readouts.
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http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/18769359http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/41256
Citation
J Acquir Immune Defic Syndr. 2008 Oct 1;49(2):123-7. doi: 10.1097/QAI.0b013e3181869a88.Publisher
University of Nairobi Department of Medical Microbiology Department of Medical Microbiology
Collections
- Faculty of Health Sciences (FHS) [10377]