Randomized comparison of different ovarian stimulation regimens for assisted reproductive technology in baboons (Papio anubis)
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Date
2011Author
Atunga, Nyachieo
Carl, Spiessens
Daniel, C. Chai
Jason, M. Mwenda
Thomas, M. d'Hooghe,
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Objective
To compare different methods of ovarian stimulation (OS) for assisted reproductive technology in baboons.
Design
Prospective randomized study.
Setting
Institute of primate research.
Animal(s)
Baboons (n = 10) were randomized into two groups (of five animals each) during three different cycles to compare six protocols of OS.
Intervention(s)
Cycle 1: clomiphene citrate (CC) alone (group CC) versus CC and GnRH agonist (group CC-Ag); cycle 2: recombinant gonadotropins (GON) without GnRH agonist (group GON) versus GON and depot GnRH agonist (group GON-AgDepo-1); cycle 3: GON and depot GnRH agonist (group GON-AgDepo-2) versus GON and daily GnRH agonist in a classic long protocol (group GON-Ag). Oocyte aspiration was performed 34–36 hours after injecting 5,000 IU rhCG, followed by fertilization via intracytoplasmic sperm injection (ICSI).
Main Outcome Measure(s)
Number and quality of oocytes retrieved and their fertilization rate.
Result(s)
More metaphase II (MII) oocytes were retrieved using the GON-AgDepo-1 (n = 12; 64% MII), GON-AgDepo-2 (n = 9; 79% MII), GON-Ag (n = 16; 88% MII), and GON (n = 6; 59% MII) protocols compared with the CC (n = 9; 15% MII) and CC-Ag (n = 14; 20% MII) protocols. Fertilization by ICSI varied between 43% and 71%.
Conclusion(s)
In baboons, long and depot protocols yield similar numbers of MII oocytes; however, depot protocol may be preferable because only one injection of GnRH agonist is needed
URI
http://www.fertstert.org/article/S0015-0282%2810%2900917-9/abstracthttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/46710
Citation
Nyachieo A, Spiessens C, Chai DC, Debrock S, Mwenda JM, D'Hooghe TM. Randomized comparison of different ovarian stimulation regimens for assisted reproductive technology in baboons (Papio anubis).. Vol. 95.; 2011. Fertil. Steril. 95(4).Publisher
University of Nairobi, College of Health Sciences,
Subject
Ovarian stimulationbaboon
ICSI
IVF
recombinant gonadotropins
agonist
antagonist
clomiphene citrate
Collections
- Faculty of Health Sciences (FHS) [10377]