Partial inactivation of CCR5- and CXCR4- tropic HIV-1 by human urine.
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Date
2011-12Author
Auvert, B
Jenabian, MA
Saidi, H
Lissouba, P
Bélec, L.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Human urine has been poorly investigated with regard to infection with human immunodeficiency virus (HIV). Here, we have studied the anti-infective functional properties of human urine against HIV. The effect of fresh urine pools on CCR5- and CXCR4-tropic HIV-1 was evaluated by using four in vitro mucosal models: reduction of infectivity of urine-treated HIV-1 particles, HIV-1 attachment to immature monocyte-derived dendritic cells (iMDDC), transfer of HIV-1 particles from iMDDC to autologous CD4 T cells, and HIV-1 transcytosis through epithelial cells. Human urine partially disrupted both CCR5- and CXCR4-tropic HIV-1 particles, moderately decreased the adsorption of HIV-1 on dendritic cells, and partially decreased the transfer of HIV-1 particles from dendritic cells to autologous T cells. These findings demonstrate partial inactivation of HIV infectivity and suggest that voiding urine after coitus could play a potential role in reducing the risk of HIV infection by both mechanically flushing out and neutralizing the infectivity of HIV-1 particles present in the genital tract.
URI
http://www.ncbi.nlm.nih.gov/pubmed/21947506http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/58230
Citation
Arch Virol. 2011 Dec;156(12):2181-6Publisher
University of Nairobi school of public health
Collections
- Faculty of Health Sciences (FHS) [10377]