Antiplasmodial Activities Of The Stem Bark Extract And Compounds Of Zanthoxylum Gilletii (De Wild) P.G. Waterman
Date
2017Author
Omosa, Leonidah K
Okemwa, Evans K
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Introduction:
Multidrug resistance remains a major obstacle hindering
successful antimalarial chemotherapy. In the current study, 50% metha
-
nol in dichloromethane extract and six compounds from the stem bark of
Zanthoxylum gilletii
were explored for their antiplasmodial potential against
three strains of
Plasmodium falciparum
.
Materials and Methods:
The ex
-
tract was obtained by cold percolation using 50 % MeOH in CH
2
Cl
2
and
the antiplasmodial activities were assayed using a non–radioactive Malaria
SYBR Green I assay to determine a concentration that inhibits growth of
50% of parasites in culture (IC
50
).
Results and Discussion:
Chromato
-
graphic separation of the crude extract yielded six known compounds
including: one lignan, sesamin (
1
), an alkamide, fagaramide (
2
), three benzo
[
c
] phenanthridine alkaloids, 8–acetonyldihydrochelerythrine (
3
), dihydro
-
chelerythine (
4
), norchelerythrine (
5
) and one pentacyclic triterpenoid,
lupeol (
6
). The extract and sesamin (
1
) showed promising antiplasmodial
activities against the chloroquine resistant (W2), chloroquine sensitive (D6)
and 3D7 strains of
P. falciparum
, with IC
50
values of 2.52, 1.48 and 1.43 μg/mL
and 5.4, 9.1 and 8.3 μM, respectively. To the best of our knowledge, this is
the first report on antiplasmodial activities of the stem bark extract (50% MeOH in CH
2
Cl
2
) and compound
1
–
3
and
6
. Furthermore, three of the
isolated compounds;
1
,
3
,
6
are reported from this species for the first
time.
Conclusion:
The good antiplasmodial activities exhibited by the stem
bark of
Z. gilletii
against three different strains of
P. falciparum
may be
attributed to the presence of
1
-
3
exhibiting good activities against all
strains of
P. falciparum
.
Citation
Omosa, Leonidah Kerubo, and Evans Kenanda Okemwa. "Antiplasmodial Activities of the Stem bark Extract and Compounds of Zanthoxylum gilletii (De wild) PG Waterman." Pharmacognosy Communications 7.1 (2017).Publisher
University of Nairobi
Rights
Attribution-NonCommercial-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-nd/3.0/us/Collections
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