Molecular surveillance of adamantane resistance among human influenza a viruses isolated in four epidemic seasons in Kenya
Date
2016Author
Wadegu, Meshack
Wamunyokoli, Fred
Osanjo, George
Opanda, Silvanos
Majanja, Janet
Rodney, Coldren
Bulimo, Wallace
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Background: Adamantanes impede influenza A virus replication and are important in the treatment and prophylaxis of disease caused by these viruses. Genotypic characterization of influenza A viruses for mutations associated with resistance to adamantanes has not been fully investigated in Kenya.
Objective: To characterize susceptibility of influenza A virus subtypes that circulated in Kenya from 2008-2011 to adamantanes.
Methods: Archived influenza A virus strains obtained from 2008 to 2011 were propagated in MDCK cells prior to sequencing of the matrix and hemagglutinin gene segments, followed by bioinformatics analyses.
Results: Ninety two virus strains consisting of 21 A/H3N2, 18 A/H1N1 and 53 A/H1N1pdm09 were analyzed. All A/H3N2 and A/H1N1pdm09 viruses displayed resistance to adamantanes due to the S31N/S31D amino acid substitution. All A/H1N1pdm09 virus strains belonged to the N-lineage characterized by S203T amino acid substitution in the HA1. All A/H1N1 viruses were sensitive to adamantane and were characterized by K140E amino acid substitution in the HA1.
Conclusion: All Kenyan influenza A/H3N2 and A/H1N1pdm09 virus strains were resistant to adamantanes while seasonal A/H1N1 strains were sensitive to these drugs. During the study period, Amantadine and Rimantadine were inappropriate for prophylaxis and treatment of influenza disease caused by A/H3N2 and A/H1N1pdm09 virus subtypes in Kenya.
Citation
Wadegu, Meshack, et al. "Molecular Surveillance of Adamantane Resistance among Human Influenza A Viruses Isolated in Four Epidemic Seasons in Kenya." African Journal of Pharmacology and Therapeutics 5.3 (2016).Publisher
University of Nairobi
Rights
Attribution-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nd/3.0/us/Collections
- Faculty of Health Sciences (FHS) [10378]
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