Identification and characterization of profilin antigen among Babesia species as a common vaccine candidate against babesiosis.
Date
2016Author
Munkhjargal, T
Aboge, GO
Ueno, A
Aboulaila, M
Yokoyama, N
Igarashi, I
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
We have characterized a member of the profilin (PROF) family protein as a common antigen in three pathogens-Babesia bovis (B. bovis), Babesia bigemina (B. bigemina), and Babesia microti (B. microti)-and evaluated its immunogenic and cross-protective properties against a challenge infection with B. microti in BALB/c mice. The recombinant PROF proteins of B. bovis, B. bigemina, and B. microti were successfully expressed in Escherichia coli (E. coli) as soluble GST fusion proteins (rBboPROF, rBbigPROF, and rBmPROF, respectively), and they were found to be antigenic. On probing with mouse anti-rPROF serum, green fluorescence was observed on the parasites' cytosols by confocal laser microscopy. Immunization regimes in BALB/c mice using rPROFs induced cross-protective immunity against B. microti infection based on high levels of cytokines and immunoglobulin (IgG) titers, a reduction in peak parasitemia levels, and earlier clearance of the parasite as compared with control mice. The findings of the present study indicate that PROF is a common antigen among bovine and murine Babesia parasites, and it might be used as a common vaccine candidate against babesiosis.
URI
https://www.ncbi.nlm.nih.gov/pubmed/27003460http://www.sciencedirect.com/science/article/pii/S0014489416300509
http://hdl.handle.net/11295/100918
Citation
Munkhjargal, Tserendorj, et al. "Identification and characterization of profilin antigen among Babesia species as a common vaccine candidate against babesiosis." Experimental parasitology 166 (2016): 29-36.Publisher
University of Nairobi
Rights
Attribution-NonCommercial-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-nd/3.0/us/Collections
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