dc.description.abstract | Conventional anti- infectives are expensive and in most cases unavailable to the target
population. This has led to greater reliance on alternative medicine such as decoctions from
plants. Lonchocarpus eriocalyx root bark, for example, has been used traditionally by many
African communities for treatment of various ailments including microbial infections.
This plant has previously been investigated for antibacterial and antiprotozooal activities.
Lupeol, a triterpenoid, has been isolated from L. eriocalyx root bark in connection to the plant’s
antiplasmodial activity. This study sought to investigate the antifungal and antibacterial activity
of the root back extracts of L. eriocalyx and to isolate more compounds responsible for activity,
with a view to giving scientific credence to the forklore use of the plant.
Lonchocarpus eriocalyx root bark was collected from Makanyanga sub-location, Igamba-
Ng’ombe division, Tharaka-Nithi County. The collected root bark was chopped into small
pieces and dried at room temperature for 2 weeks. The dried plant material was ground to
powder and stored at room temperature before use.
Sequential extraction was carried out using methanol, chloroform and water. All the four extracts
were screened for antibacterial activity against Staphylococcus aureus, Escherichia coli and
Pseudomonas aeruginosa, and for antifungal activity against Saccharomyces cerevisiae using
agar diffusion method. Gentamicin and nystatin were used as positive controls for antibacterial
and antifungal tests respectively.
Four compounds were isolated by column chromatography in a gradient mode using silica gel
60-120 mesh, monitored by TLC. They were identified as β- sitosterol/stigmasterol mixture,
lupenone, and lupeol using UV, IR, MS and NMR analysis. This is the first time that β-
sitosterol, stigmasterol and lupenone have been isolated from L. eriocalyx.
The four compounds exhibited antibacterial activity against S. aureus, E. coli and P. aeruginosa,
and antifungal activity against S. cerevisiae. The greatest activity was against S. cerevisiae, with
zones on inhibition of 1.1 cm, 1.2 cm and 1.3 cm for β-sitosterol/stigmasterol mixture, lupenone
and lupeol respectively. This gives scientific credence to the forklore use of the root bark of
Lonchocarpus eriocalyx in the treatment of bacterial and fungal infections. | en_US |
dc.description.department | a
Department of Psychiatry, University of Nairobi, ; bDepartment of Mental Health, School of Medicine,
Moi University, Eldoret, Kenya | |