Examination of alkaloidal constituents of zanthoxylum usambarense by a combination of ion-pair extraction and ion-pair chromatography using sodium perchlorate
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Date
1995Author
Kato, Atsushi
Moriyasu, Masataka
Ichimaru, Momoyo
Nishiyama, Yumi
Juma, Francis D.
Nganga, Joseph N
Mathenge, Simon G.
Ogeto, John O.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Quaternary alkaloids of the stem of Zanthoxylum usambarense, an important medicinal plant in Kenya utilized for the treatment of pneumonia and rheumatism, were examined by a combination of ion-pair extraction and preparative ion-pair high performance liquid chromatography (HPLC), in which sodium perchlorate was used as an ion-pair reagent. Quaternary bases were extractable from aqueous solution into I ,2-dichloroethane in the presence of perchlorate in acidic conditions. The phenomenon is explained in terms of ion-pair extraction between alkaloidal cation and perchlorate anion. The quaternary alkaloidal fraction thus obtained was well separated by means of ion-pair HPLC in which sodium perchlorate was also utilized. Furthermore, the solvent system was easily applied to preparative HPL " yielding the pure perchlorate of each alkaloid. Eight quaternary bases in Z. usambarense were thus obtained and their structures were elucidated. One of them wa a new quaternary base of the tetrahydroproloberberine type, which was named (-)-usambarine.
Citation
Phytochemical analysis Vol. 6Publisher
Department of Natural Medicinal Chemistry, Kobe Pharmaceutical University Department of Medicine, University of Nairobi Department of Botany, University of Nairobi Department of Pharmacy, University of Nairobi
Subject
Ion-pair extraction;ion-pair high performance liquid chromatography;
sodium perchlorate;
Quaternary alkaloids;
(- )-usambarine;
Zanthoxylum usambarense .