dc.contributor.author | Aboge, Gabriel Oluga | |
dc.contributor.author | Jia, Honglin | |
dc.contributor.author | Goo, Younkyoung | |
dc.contributor.author | Kuriki, Ken | |
dc.contributor.author | Nishikawa, Yoshifumi | |
dc.contributor.author | Igarashi, Ikuo | |
dc.contributor.author | Suzuki, Hiroshi | |
dc.contributor.author | Xuan, Xuenan | |
dc.date.accessioned | 2013-02-26T09:50:57Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Veterinary Parasitology xxx (2007) xxx-xxx | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/11606 | |
dc.description.abstract | We isolated a novel single copy gene encoding a 57-kDa merozoite protein of Babesia gibsoni (BgP57). The nucleotide sequence of the cDNA was 2387 bp with an open reading frame (ORP) of 1644 bp encoding a 57-kDa predicted polypeptide having 547 amino acid residues. The recombinant BgP57 (rBgP57) without a predicted signal peptide was expressed in Escherichia coli as a soluble glutathione S-transferase (GST) fusion protein. Western blotting showed that the corresponding native protein was 57-kDa, consistent with molecular weight of predicted mature polypeptide. An indirect enzyme-linked immunosorbent assay (ELISA) using the rBgP57 detected specific antibodies in the sequential sera from a dog experimentally infected with B. gibsoni. Moreover, the antigen did not cross-react with antibodies to B. canis sub-species and closely related apicomplexan parasites indicating that the rBgP57 was a specific antigen for B. gibsoni antibodies. The diagnostic performance of ELISA based on rBgP57 using 107 sera from B. gibsoni-naturally infected dogs was the same as the previously identified rBgP32 but performed better than the previously studied rBgP50. Although, seminested peR detected higher proportions (82%) of positive samples than the ELISAs, the Mcnemar's chi-square test showed that there was no significant difference in relative effectiveness of rBgP57-ELISA and seminested peR (x2 = 2.70; P = 0.1003) in identifying positive samples. The rBgP57-ELISA when used in combination with rBgP32-ELISA and rBgP50-ELISA appeared to improve sensitivity of the rBgP57-ELISA for detection of B. gibsoni antibodies. Overall, the rBgP57-ELISA and seminested peR when used in combination, could improve epidemiological surveys and clinical diagnosis of B. gibsoni infection. | en |
dc.language.iso | en | en |
dc.subject | Elisa | en |
dc.subject | 57-kDa merozoite | en |
dc.subject | Protein; B. gibsoni | en |
dc.title | A novel 57-kDa merozoite protein of Babesia gibsoni is a prospective antigen for diagnosis and serosurvey of canine babesiosis by enzyme-linked immunosorbent assay | en |
dc.type | Article | en |
local.publisher | Department of Public Health Pharmacology and Toxicology | en |