dc.contributor.author | Matheka, Duncan M | |
dc.contributor.author | Kitua, Morris | |
dc.contributor.author | Alkizim, Faraj O. | |
dc.date.accessioned | 2013-04-04T11:34:28Z | |
dc.date.available | 2013-04-04T11:34:28Z | |
dc.date.issued | 2012 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/15333 | |
dc.description.abstract | Background: Laboratory investigations on diabetes mellitus (DM) have employed various animal models. Alloxan, a β-cell toxin, has widely been used to induce DM in animals. The current study reports peculiar glycemic patterns during alloxan DM induction.
Method: Thirty eight, six-month old Sprague Dawley rats were assigned to two groups: control (n=14) and experimental (n=24). Alloxan (125 mg/kg) was administered intraperitoneally to the experimental group, and an equal volume of normal saline to the control. Using a glucometer, fasting blood glucose (FBG) levels were assessed on
days 0, 3, 6, 13, 20 and 27.
Results: At day 3, FBG levels were higher in the experimental group (19.26±1.77 vs. 3.21±0.04 mmol/l, p<0.01). Thereafter, FBG levels in the experimental group reduced gradually till day 27, though remaining higher than the control (7.59±0.86 vs. 3.22±0.13 mmol/l, p<0.01). The FBG levels in the control oscillated between 3.14±0.04 and
3.26±0.05 mmol/l. Out of the 24 rats administered with alloxan, only 10 were diabetic at day 27.
Conclusion: Induction of diabetes in rats using alloxan (125mg/kg) leads to variability in glucose levels. A number of animals greater than required may be necessary to accommodate failed diabetes induction. Follow-up for at least one month is necessary before confirming induction. | en |
dc.language.iso | en | en |
dc.publisher | African Journal of Pharmacology and Therapeutics | en |
dc.relation.ispartofseries | Vol. 1 No. 1;Pages 30-34 | |
dc.subject | animal model, alloxan, diabetes induction | en |
dc.title | Peculiar glycemic patterns in alloxaninduced diabetes animal model | en |
dc.type | Article | en |
dc.description.department | a
Department of Psychiatry, University of Nairobi, ; bDepartment of Mental Health, School of Medicine,
Moi University, Eldoret, Kenya | |
local.publisher | Department of Medical Physiology | en |