Use of Repetitive Extragenic Palindromic (REP), Enterobacterial Repetitive Intergenic Consensus (ERIC) and BOX sequences to fingerprint Exserohilum turcicum isolates
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Date
2010-04Author
Muiru, W M
Koopmann, B
Tiedemann, A V
Mutitu, E W
Kimenju, J W
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Objective: To examine the distribution of dispersed repetitive DNA (Enterobacterial Repetitive Intergenic
Consensus (ERIC), BOX, and Repetitive Extragenic Palindromic (REP) sequences in the genome of
Exserohilum turcicum using conserved primers.
Methodology and results: DNA amplification was done using standard PCR procedures and the resultant
PCR products analyzed by agarose and polyacrylamide gel electrophoresis. The different markers
produced characteristic banding patterns and the similarity matrices from binary banding data was derived
with the similarity for qualitative data program (SIMQUAL). Cluster analysis showed close genetic
similarities between Kenyan, German and Austrian isolates of E. turcicum. The average gene diversity from
BOX sequences was 0.28 whereas for ERIC and REP sequences, it was 0.14 and 0.05, respectively.
Conclusion and application of findings: The study showed that discrimination of closely related strains can
be achieved using REP, BOX and ERIC PCR fingerprinting. We recommend these markers as a rapid tool
for the genetic characterization of large populations of E. turcicum.
Citation
Journal of Applied Biosciences 30: 1828 - 1838Publisher
Department of Plant Science and Crop Protection, University of Nairobi
Subject
Repetitive Extragenic PalindromicEnterobacterial Repetitive Intergenic Consensus
BOX sequences
Fingerprint Exserohilum turcicum isolates
Description
Journal article