dc.contributor.author | Muiru, W M | |
dc.contributor.author | Koopmann, B | |
dc.contributor.author | Tiedemann, A V | |
dc.contributor.author | Mutitu, E W | |
dc.contributor.author | Kimenju, J W | |
dc.date.accessioned | 2013-04-15T11:34:36Z | |
dc.date.issued | 2010-04 | |
dc.identifier.citation | Journal of Applied Biosciences 30: 1828 - 1838 | en |
dc.identifier.issn | 1997-5902 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/16004 | |
dc.description | Journal article | en |
dc.description.abstract | Objective: To examine the distribution of dispersed repetitive DNA (Enterobacterial Repetitive Intergenic
Consensus (ERIC), BOX, and Repetitive Extragenic Palindromic (REP) sequences in the genome of
Exserohilum turcicum using conserved primers.
Methodology and results: DNA amplification was done using standard PCR procedures and the resultant
PCR products analyzed by agarose and polyacrylamide gel electrophoresis. The different markers
produced characteristic banding patterns and the similarity matrices from binary banding data was derived
with the similarity for qualitative data program (SIMQUAL). Cluster analysis showed close genetic
similarities between Kenyan, German and Austrian isolates of E. turcicum. The average gene diversity from
BOX sequences was 0.28 whereas for ERIC and REP sequences, it was 0.14 and 0.05, respectively.
Conclusion and application of findings: The study showed that discrimination of closely related strains can
be achieved using REP, BOX and ERIC PCR fingerprinting. We recommend these markers as a rapid tool
for the genetic characterization of large populations of E. turcicum. | en |
dc.language.iso | en | en |
dc.subject | Repetitive Extragenic Palindromic | en |
dc.subject | Enterobacterial Repetitive Intergenic Consensus | en |
dc.subject | BOX sequences | en |
dc.subject | Fingerprint Exserohilum turcicum isolates | en |
dc.title | Use of Repetitive Extragenic Palindromic (REP), Enterobacterial Repetitive Intergenic Consensus (ERIC) and BOX sequences to fingerprint Exserohilum turcicum isolates | en |
dc.type | Article | en |
local.publisher | Department of Plant Science and Crop Protection, University of Nairobi | en |