dc.contributor.author | Willim, Rachel | |
dc.contributor.author | Shadabi, Elnaz | |
dc.contributor.author | Sampathkumar, Raghavan | |
dc.contributor.author | Lin, Li | |
dc.contributor.author | Balshaw, Robert | |
dc.contributor.author | Kimani, Joshua | |
dc.contributor.author | Plummer, Francis A | |
dc.contributor.author | Luo, Ma | |
dc.contributor.author | Liang, Binhua | |
dc.date.accessioned | 2022-07-07T04:40:59Z | |
dc.date.available | 2022-07-07T04:40:59Z | |
dc.date.issued | 2022-01 | |
dc.identifier.citation | Willim R, Shadabi E, Sampathkumar R, Li L, Balshaw R, Kimani J, Plummer FA, Luo M, Liang B. High Level of Pre-Treatment HIV-1 Drug Resistance and Its Association with HLA Class I-Mediated Restriction in the Pumwani Sex Worker Cohort. Viruses. 2022 Jan 28;14(2):273. doi: 10.3390/v14020273. PMID: 35215866; PMCID: PMC8879707. | en_US |
dc.identifier.uri | https://pubmed.ncbi.nlm.nih.gov/35215866/ | |
dc.identifier.uri | http://erepository.uonbi.ac.ke/handle/11295/161246 | |
dc.description.abstract | Background: We analyzed the prevalence of pre-antiretroviral therapy (ART) drug resistance mutations (DRMs) in a Kenyan population. We also examined whether host HLA class I genes influence the development of pre-ART DRMs.
Methods: The HIV-1 proviral DNAs were amplified from blood samples of 266 ART-naïve women from the Pumwani Sex Worker cohort of Nairobi, Kenya using a nested PCR method. The amplified HIV genomes were sequenced using next-generation sequencing technology. The prevalence of pre-ART DRMs was investigated. Correlation studies were performed between HLA class I alleles and HIV-1 DRMs.
Results: Ninety-eight percent of participants had at least one DRM, while 38% had at least one WHO surveillance DRM. M184I was the most prevalent clinically important variant, seen in 37% of participants. The DRMs conferring resistance to one or more integrase strand transfer inhibitors were also found in up to 10% of participants. Eighteen potentially relevant (p < 0.05) positive correlations were found between HLA class 1 alleles and HIV drug-resistant variants.
Conclusions: High levels of HIV drug resistance were found in all classes of antiretroviral drugs included in the current first-line ART regimens in Africa. The development of DRMs may be influenced by host HLA class I-restricted immunity. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.subject | antiretroviral therapy; drug-resistant mutation; human immunodeficiency virus; human leukocyte antigen; next-generation sequencing technology. | en_US |
dc.title | High Level of Pre-Treatment HIV-1 Drug Resistance and Its Association with HLA Class I-Mediated Restriction in the Pumwani Sex Worker Cohort | en_US |
dc.type | Article | en_US |