A specific enzyme-linked immunosorbent assay (ELISA) procedure for detection of heterophile Hanganutziu and Deicher (HD) antibodies
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Date
1986-02-12Author
Mukuria, J C
Naiki, M
Hashimoto, M
Kato, S
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
A specific, relatively sensitive, quantitative and standardized enzyme-linked immunosorbent assay (ELISA) procedure was developed for the detection of heterophile Hanganutziu and Deicher (HD) antibodies which are occasionally elevated in pathologic human sera. The HD antigen-active molecule used for the assay was a ganglioside (N-glycolylneuraminyllactosylceramide, abbreviated as NeuGc-LacCer) previously purified from horse erythrocyte membranes. The test used antigen-coated plastic microtiter plates and anti-human immunoglobulin G (IgG, Fab fragment) conjugated with alkaline phosphatase. Fifty-four normal human sera gave ELISA values ranging from −2 to 2%. Random sera from hospitalized patients were first screened by the horse erythrocyte hemagglutination (HA) test, whereby 5.7% (76 cases) gave abnormal HA titers of 128–4096 compared to titers in normal sera equal to or less than 64. Ninety-seven % of the patients' sera gave abnormal ELISA values (3–200%). They were classified into 3 groups: cancer (42 cases), infection (10 cases), and others (24 cases). The potential value of this ELISA method is discussed.
URI
http://www.sciencedirect.com/science/article/pii/0022175986904503http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/16532
Citation
Journal of immunological methods, 86(2): 179-185Publisher
College of Health Sciences, University of Nairobi
Subject
ELISA;Hanganutziu and Deicher antibodies
Hemagglutination
Heterophile
N-glycolylneuraminic acid
Description
Journal article
Collections
- Faculty of Health Sciences (FHS) [10378]