dc.contributor.author | Okumu, K'Aluoch F | |
dc.date.accessioned | 2013-05-12T11:41:49Z | |
dc.date.available | 2013-05-12T11:41:49Z | |
dc.date.issued | 1994 | |
dc.identifier.citation | Master of Science | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/22495 | |
dc.description.abstract | Immunotherapy of post-surgical Alveolar Hydatid Disease (AHD) was
attempted using an attenuated strain of Mycobacterium bovis (BCG) in this study.
Gerbils were subcutaneously infected with Echinococcus multilocularis. Ninety days
later the established subcutaneous cyst mass was surgically removed in three groups of
gerbils that had been administered with either BCG (a macrophage activator),
Carrageenan (CAR) (an anti-macrophage agent) or saline 10-12 days before the
operation. In the fourth group of gerbils the established cyst was left intact. At
necropsy 30 and 60 days post-surgery, body regions with disseminated cyst foci were
recorded and metastatic cyst weights taken in all the experimental groups. Cyst tissue
samples were taken for histology and blood smears were examined for differential
white blood cell counts.
Metastasis occurred mostly to the draining lymph nodes suggesting that
germinal tissue was disseminated mostly via the lymphatic vessels.
All four treatments groups did not show any significant difference in their
levels of metastasis. Histological appearance of the qualitative leucocyte cellular
response towards the parasite was similar in all the groups.
It is suggested that BCG does not have an enhancing effect on macrophages
already suppressed by the chronic parasitic infection. In fact macrophages may be
further suppressed, thus not controlling post-surgical growth of metastatic foci. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | Immunotherapy of post-surgical alveolar hydatid disease (AHD) | en |
dc.type | Thesis | en |
local.publisher | THE UNIVERSITY OF NEW BRUNSWICK, Department Of Biology | en |