| dc.description.abstract | A technique for the analysis of some common natural fatty acids
has been developed by the use of reverse-phase high performance
liquid chromatography (RPLC) .
The fatty acid standards were derivatised with 1?_-phenylphenacyl
bromide via the potassium salt or using tetra--n-butylammonium hydrogensulphate
as phase-transfer catalyst. The £-phenylphenacyl esters
of dodecanoic (c12 :0), tetradecanoic (c14 :0), hexadecanoic (c16 :0),
Z-9-hexadecenoic (c 16 : l;gz), octadecanoic (ClB :O)' Z-9-octadecenoic
(ClB :l; 9Z), E-9-octadecenoic (ClS : l; 9E), Z-9, Z-12-octadecadienoj _
(c18 :2; 9Z,l 22), Z-9, Z-12, Z-15-octadecatrienoic (ClB :J;gz,l 2Z,lSZ),
12-hydroxy-Z-9-octadecenoic (ClB:l ;
9Z(l20H)) and Z-13-docosenoic
(c22 :l;lJZ) acids were synt tesized and characterized . The last five
esters have not been synthesized previously.
These fatty acid es er derivatives were then chromatographed
on a 5u ult r a phere ODS column using various mobile phases , including
absolute methanol, methanol-water, methanol-acetonit rile and
methanol-acetonitrile-water m·xtures. The best mobile phase was
found to be a mixture of methanol , acetonitrile and water , which
made it possible to resolve all of the critical pairs (£-phenylphenacyl
ester derivatives of hexadecanoic (c16 :0)
& Z- 9- octadecenoic...... | English |
