dc.contributor.author | Keter, Lucia K | |
dc.date.accessioned | 2013-06-07T09:28:11Z | |
dc.date.available | 2013-06-07T09:28:11Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Keter, L.K(2005). Development of A Liquid Chromatographic | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/11295/29929 | |
dc.description | Master of pharmacy Thesis | en |
dc.description.abstract | A liquid chromatographic method for the simultaneous determination of six HIV -protease
inhibitors, indinavir, saquinavir, ritonavir, amprenavir, nelfinavir and lopinavir, was
developed and validated.
The method was developed by systematic investigation of the various stationary phases
and the effects of the pH, various organic modifiers, ionic strength and the column
temperature on chromatographic parameters. Optimal separation was achieved with a
PLRP-S 100 A 250 x 4.6 mm LD. column maintained at 60°C, the mobile phase
consisted of tetrahydrofuran-O.1 M potassium phosphate buffer pH 5.0-0.1 M
tetrabutylammonium hydrogen sulphate pH 5.0-water (35:30:10:25, v/v) at a flow rate of
1.0 ml per minute and ultraviolet detection at 254 nm.
The liquid chromatography method was validated by determining the linearity of detector
response, precision, limit of detection and limit of quantitation, stability and ruggedness.
The method was found to be linear over the specific ranges investigated and the R values
were between 0.9997 and 0.9915 for the six drugs. The limit of quantitation for the six
drugs was 160 to 5120 ng, while the limit of detection was 80 to 2120 ng. The intra-day
and inter-day precision was within the ranges of 0.39 to 1.79 % and 0.55 to 7.46 %,
respecti vely.
The method was used to evaluate the content of active pharmaceutical ingredient of some
protease inhibitors in the Kenyan market.
The method developed can be used in routine quantitative analysis of the HIV -protease
inhibitors in commercial samples and bulk pharmaceutical raw materials. | en |
dc.language.iso | en | en |
dc.subject | Liquid Chromatographic | en |
dc.subject | Simultaneous Analysis | en |
dc.subject | Protease Inhibitors | en |
dc.title | Development of a liquid chromatographic method for the simultaneous analysis of six protease inhibitors | en |
dc.type | Thesis | en |
dc.description.department | a
Department of Psychiatry, University of Nairobi, ; bDepartment of Mental Health, School of Medicine,
Moi University, Eldoret, Kenya | |
local.publisher | Department of Pharmaceutical Chemistry, University of Nairobi | en |