dc.contributor.author | Mwangi, DM | |
dc.contributor.author | Honda, Y | |
dc.contributor.author | Graham, SP | |
dc.contributor.author | Pelle, R | |
dc.contributor.author | Taracha, EL | |
dc.contributor.author | Gachanja, J | |
dc.contributor.author | Nyanjui, JK | |
dc.contributor.author | Bray, J | |
dc.contributor.author | Palmern, GH | |
dc.contributor.author | Brown, WC | |
dc.contributor.author | Mwagi, W | |
dc.date.accessioned | 2013-06-13T13:05:32Z | |
dc.date.available | 2013-06-13T13:05:32Z | |
dc.date.issued | 2011-04 | |
dc.identifier.citation | Vet Immunol Immunopathol. 2011 Apr 15;140(3-4):244-51 | en |
dc.identifier.uri | http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/21288576 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/33180 | |
dc.description.abstract | Theileria parva antigens recognized by cytotoxic T lymphocytes (CTLs) are prime vaccine candidates against East Coast fever in cattle. A strategy for enhancing induction of parasite-specific T cell responses by increasing recruitment and activation of dendritic cells (DCs) at the immunization site by administration of bovine Flt3L and GM-CSF prior to inoculation with DNA vaccine constructs and MVA boost was evaluated. Analysis of immune responses showed induction of significant T. parva-specific proliferation, and IFN-γ-secreting CD4(+) and CD8(+) T cell responses in immunized cattle. However, antigen-specific CTLs were not detected. Following lethal challenge, 5/12 immunized cattle survived by day 21, whereas all the negative controls had to be euthanized due to severe disease, indicating a protective effect of the vaccine (p<0.05). The study demonstrated the potential of this technology to elicit significant MHC class II and class I restricted IFN-γ-secreting CD4(+) and CD8(+) T cells to defined vaccine candidate antigens in a natural host, but also underscores the need to improve strategies for eliciting protective CTL responses. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi. | en |
dc.title | Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses | en |
dc.type | Article | en |
local.publisher | Department of Veterinary Pathology and Microbiology, University of Nairobi, Kenya. | en |