Characterization of a leucine aminopeptidase of Babesia gibsoni
dc.contributor.author | Jia, H | |
dc.contributor.author | Terkawi, M. A | |
dc.contributor.author | Aboge, G. O | |
dc.contributor.author | Goo, Y.K | |
dc.contributor.author | Luo, Y | |
dc.contributor.author | Li, Y | |
dc.contributor.author | Yamagishi, J | |
dc.contributor.author | Nishikawa, Y | |
dc.contributor.author | Igarashi, I | |
dc.contributor.author | Sugimoto, C | |
dc.contributor.author | Fujisaki, K | |
dc.contributor.author | Xuan, X | |
dc.date.accessioned | 2013-06-18T15:25:08Z | |
dc.date.available | 2013-06-18T15:25:08Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | H. Jia, M. A. Terkawi, G. O. Aboge et al (2009). Characterization of a leucine aminopeptidase of Babesia gibsoni. Parasitology / Volume 136 / Issue 09 / August 2009, pp 945-952 | en |
dc.identifier.uri | http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=5918112 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/35874 | |
dc.description.abstract | Peptidases of parasitic protozoa are currently under intense investigation in order to identify novel virulence factors, drug targets, and vaccine candidates, except in Babesia. Leucine aminopeptidases in protozoa, such as Plasmodium and Leishmania, have been identified to be involved in free amino acid regulation. We report here the molecular and enzymatic characterization, as well as the localization of a leucine aminopeptidase, a member of the M17 cytosolic aminopeptidase family, from B. gibsoni (BgLAP). A functional recombinant BgLAP (rBgLAP) expressed in Escherichia coli efficiently hydrolysed synthetic substrates for aminopeptidase, a leucine substrate. Enzyme activity of the rBgLAP was found to be optimum at pH 8·0 and at 37°C. The substrate profile was slightly different from its homologue in P. falciprum. The activity was also strongly dependent on metal divalent cations, and was inhibited by bestatin, which is a specific inhibitor for metalloprotease. These results indicated that BgLAP played an important role in free amino acid regulation. | en |
dc.language.iso | en | en |
dc.title | Characterization of a leucine aminopeptidase of Babesia gibsoni | en |
dc.type | Article | en |
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