Rabies-specific production of interleukin-2 by peripheral blood lymphocytes from human rabies vaccinees
View/ Open
Date
1991-08Author
Perrin P.
Joffret ML.
Zanetti C.
Bourhy, H
Gontier Christopher Stuart.
Fritzell C.
Leclerc C.
Sureau P.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Cell-mediated immunity induced by rabies vaccination was studied in humans by the determination of specific interleukin-2 (IL-2) production in a large number of donors (postexposure immunized patients and pre-exposure immunized laboratory workers). Peripheral blood lymphocytes (PBL) from 35 donors were tested for IL-2 production after in vitro stimulation by different rabies and rabies-related viruses. IL-2 responses were compared to antibody recognition of these different virus serotypes by sera from the same individuals. IL-2 was produced by PBL from more than 85% of donors after stimulation with inactivated and purified rabies viruses (IPRV) prepared from either Pittman Moore (PM) or Pasteur Virus (PV) strains. IL-2 was also produced by 65 and 45% of donor PBL stimulated with IPRV from the European Bat Lyssavirus (EBL) and Mokola (Mok) rabies-related virus strains respectively. No correlation was found between the production of IL-2 by PBL and the levels of virus neutralizing antibody (VNAb). Moreover, 50, 25 and 35% of donors produced IL-2 after stimulation of their PBL with ribonucleoprotein (RNP) from PV-, EBL- and Mok-viruses, respectively. These results obtained with a large number of human rabies vaccinees and using an assay specific to T-cell activation confirm the significant cross-reactivity of T-cell responses directed against rabies and rabies-related viruses. This study shows that IL-2 production could be used for the study of cell-mediated immunity and T-cell memory induced in humans by rabies vaccination.
URI
http://profiles.uonbi.ac.ke/christophergontier/publications/perrin-p-joffret-ml-zanetti-c-bourhy-h-gontier-c-fritzell-c-leclerc-http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/38408
http://www.ncbi.nlm.nih.gov/pubmed/1771968
Citation
STUART, DRGONTIERCHRISTOPHER. 1991. Perrin P, Joffret ML, Zanetti C, Bourhy H, Gontier C, Fritzell C, Leclerc C, Sureau P. Rabies-specific production of interleukin-2 by peripheral blood lymphocytes from human rabies vaccinees.Vaccine. 1991 Aug;9(8):549-58.. Vaccine. 1991 Aug;9(8):549-58..Publisher
Department of Human Pathology, College of Health Sciences, University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10378]