Factors affecting exflagellation of in-vitro cultivated Plasmodium falciparum gametocytes
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Date
1993Author
OGWAN'G, R. A
MWANGI, J. K
GITHURE, J
WERE, J. B. O
ROBERTS, C. R
MARTIN, SK
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The environment of Plasmodium falciparum gametocytes changes when they make the transition from the vertebrate to the invertebrate host. Gametocytes of this species cultivated in vitro were used to evaluate the effect ofserum, pH, pCO2 tension, bicarbonate ion, and temperature on gamete formation. Temperature was the only factor responsible for keeping P. falciparum gametocytes in the inactivated state. Mature gametocytes held at temperatures above 30oC remained quiescent in 10% serum, even at low ambient pCO2 tension, alkaline pH, and in the presence of 23 mM bicarbonate ion. When the temperature of the medium was allowed to drop below 30oC, gametocytes emerged from the red blood cells and microgametocytes consistently exflagellated at pH 7.4, even in the absence of bicarbonate ion
URI
http://cat.inist.fr/?aModele=afficheN&cpsidt=3784752http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/42051
Citation
The American journal of tropical medicine and hygiene 1993, vol. 49, no1, pp. 25-29Publisher
University of Nairobi