A potential radioimmunoassay system for detection of Hanganutziu-Deicher type heterophile antigen (s) and antibodies in tissues and fluids
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Date
1985-06Author
Kato, Shiro
Okabe, Masahiro
Nishiura, Katsumi
Hashimoto, Masato
Naiki, Masaharu
Mukuria, Joseph C
Type
ArticleLanguage
enMetadata
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A relatively simple, specific and sensitive radioimmunoassay system has been developed for the detection of heterophile Hanganutziu-Deicher (H-D) antigen(s) and antibodies. The 125I-labeled H-D antigen-active molecule used for the assay is a bovine erythrocyte major glycoprotein found to have a strong H-D antigen potency. The antigen-antibody complex was precipitated with normal human serum as the carrier protein, followed by the addition of rabbit anti-human IgG F(ab')2 serum. With this method, different H-D antigen-active molecules were compared for heterophile H-D antigen potency with reasonable sensitivity detecting about 0.3 ng of cold glycoprotein. 8 different lung cancer tissues were assayed for H-D antigen. The sera from the 8 lung cancer patients were also screened by ELISA and RIA in an attempt to correlate expression of H-D antigen on tissues with elevation of H-D antibodies. The results showed that all patients' tissues expressed the antigen(s) but only 3 of them had abnormal levels of H-D antibodies. This could have been due to excess antigens in circulation or immune complexes
Citation
Journal of Immunological Methods Volume 80, Issue 1, 12 June 1985, Pages 97–106Publisher
Department of Biochemistry
Collections
- Faculty of Health Sciences (FHS) [10378]