Separate and combined effects of caffeine and dbcAMP on olive baboon (Papio anubis) sperm
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Date
2010-06Author
Atunga, Nyachieo
Carl, Spiessens
Chai2, Nicholas M. Kiulia2, Jason M. Mwenda2, Thomas M. D’Hooghe
Daniel, C Chai
Nicholas, M. Kiulia
Jason, M. Mwenda
Thomas, M. D’Hooghe
Type
ArticleLanguage
enMetadata
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Background Improvement of baboon sperm capacitation is necessary for achieving high in vitro fertilization (IVF) rates in baboons. In this study, we evaluated separate and combined effects of caffeine and dbcAMP on baboon sperm capacitation.
Methods Sixteen male baboons (n = 16) were electroejaculated. Each sperm sample was divided into two aliquots: one for chemical activation and the other untreated control. Group 1: dbcAMP (n = 6); Group 2: caffeine (n = 6) and Group 3: combination of caffeine and dbcAMP (n = 4). In each aliquot, sperm motility after 30 minutes of incubation was evaluated as well as zona pellucida (ZP) binding ability after overnight incubation with 4–5 ZP from unfertilized human oocytes.
Results Sperm motility and ZP binding ability in all chemically activated groups increased significantly as compared to their respective controls (P < 0.05).
Conclusion Combined and separate effects of caffeine and dbcAMP increases baboon sperm motility and ZP binding ability and may improve baboon IVF.
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http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0684.2010.00401.x/abstract?deniedAccessCustomisedMessage=&userIsAuthenticated=falsehttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/46695
Citation
Nyachieo A, Spiessens C, Chai DC, Kiulia NM, Mwenda JM, D'Hooghe TM. Separate and combined effects of caffeine and dbcAMP on olive baboon (Papio anubis) sperm.. Vol. 39.; 2010. J. Med. Primatol. 39(3).Publisher
University of Nairobi, College of Health Sciences,
Collections
- Faculty of Health Sciences (FHS) [10378]