Comparison of antigenicity of toxins produced by Clostridium botulinum type C and D strains.
Date
1984Author
Ochanda, J.O.
Syuto, B
Oguma, K.
Iida, H.
Kubo, S.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
C1 neurotoxin of Clostridium botulinum strains C-Stockholm (C-ST), C beta-Yoichi, C-468, CD6F, and C-CB19 and type D toxin of strains D-1873 and D-CB16 were purified by gel filtration, ion exchange, and affinity chromatographies. The purified toxins had di-chain structure made of heavy and light chains. The toxins of C beta-Yoichi, C-468, CD6F, and C-CB19 reacted with anti-C-ST heavy chain and anti-C-ST light chain in immunodiffusion tests and enzyme-linked immunosorbent assay, whereas D-CB16 toxin reacted with anti-D-1873 heavy chain and anti-D-1873 light chain. However, C-6813 toxin reacted with anti-D-1873 heavy chain and anti-C-ST light chain but not with anti-C-ST heavy chain or anti-D-1873 light chain immunoglobulin G. These results indicate common antigens in the heavy chains of C-6813 and D-1873 toxins and in the light chains of C-6813 and C-ST toxins. Further, they provide evidence for heterogeneity within type C1 toxin subunits.
Citation
O., PROFOCHANDAJAMES. 1984. Ochanda JO, Syuto B, Oguma K, Iida H, Kubo S.Comparison of antigenicity of toxins produced by Clostridium botulinum type C and D strains.Appl Environ Microbiol. 1984 Jun;47(6):1319-22. Appl Environ Microbiol. 1984 Jun;47(6):1319-22. : East African Medical JournalPublisher
University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10387]