Determination of human herpes simplex virus in clear cerebrospinal fluids by multiplex polymerase chain reaction (PCR) in Rwanda
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Date
2013Author
Ntagwabira, Edouard
Mwaengo, Dufton
Flandin, Jean-Frederic
Muhizi, Charles
Mukabayire, Odette
Anzala, Omu
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The purpose of this study was to test CSF obtained from different regions of Rwanda for herpes simplex viruses (HSV) type 1 and 2 using a commercial multiplex PCR kit. CSF samples were obtained from patients with clinical suspicion of meningitis and encephalitis which may be caused by different microorganisms including herpes viruses. More than 80% of clear cerebrospinal fluid (CSF) submitted to two referral hospitals (King Faisal Hospital and University Teaching Hospital of Kigali) and to the National Reference Laboratory of Rwanda, were found to be negative for Cryptococcus neoformans and Mycobacterium tuberculosis, the only microorganisms tested at these facilities.
Citation
African Journal of Biotechnology Vol. 12(19), pp. 2553-2557, 8 May, 2013Publisher
Department of Medical Microbiology
Subject
RwandaMycobacterium tuberculosis
Cryptococcus neoformans
encephalitis
meningitis
multiplex polymerase chain reaction (PCR)
cerebrospinal fluids
herpes simplex virus 2 (HSV2)
Herpes simplex virus 1 (HSV1)
Collections
- Faculty of Health Sciences (FHS) [10387]