Isocratic liquid chromatographic method for the analysis of azithromycin and its structurally related substances in bulk samples.
View/ Open
Date
2002Author
Hoogmartens, J
Roets, E
Debremaeker, D
Ngugi, JK
Chepkwony, HK
Kamau, Franco N.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
An isocratic liquid chromatographic method with UV detection at 215 nm, which is suitable for the analysis of azithromycin (AZT) in bulk samples, is described. AZT is separated from its synthesis intermediates and a degradation product as well as from six unknown impurities on an XTerra RP18 column at 70 degrees C using a mobile phase consisting of acetonitrile-pH 6.5 0.2M K2HPO4-water (35:10:55, v/v/v) at 1.0 mL/min. The XTerra stationary phase contains methyl groups that are incorporated in the bulk structure of the material. This allows for special selectivities. Robustness is evaluated by a full factorial design experiment. The method shows good selectivity, repeatability, linearity, and sensitivity.
URI
http://www.ncbi.nlm.nih.gov/pubmed/12433117http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/47884
Citation
J Chromatogr Sci. 2002 Oct;40(9):529-33.Publisher
Department of Pharmaceutical Chemistry
Collections
- Faculty of Health Sciences (FHS) [10387]