Diagnosis of Burkitt lymphoma using an algorithmic approach - applicable in both resource-poor and resource-rich countries.
Date
2011-07Author
Naresh KN.
Ibrahim HA.
Lazzi S.
Rince P.
Onorati M.
Ambrosio MR.
Bilhou-Nabera C.
Amen F.
Reid A.
Mawanda M.
Calbi V.
Ogwang M.
Rogena Emily Adhiambo.
Byakika B.
Sayed S.
Moshi E.
Mwakigonja A.
Raphael M.
Magrath I.
Leoncini L.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Distinguishing Burkitt lymphoma (BL) from B cell lymphoma, unclassifiable with features intermediate between diffuse large B-cell lymphoma (DLBCL) and BL (DLBCL/BL), and DLBCL is challenging. We propose an immunohistochemistry and fluorescent in situ hybridization (FISH) based scoring system that is employed in three phases - Phase 1 (morphology with CD10 and BCL2 immunostains), Phase 2 (CD38, CD44 and Ki-67 immunostains) and Phase 3 (FISH on paraffin sections for MYC, BCL2, BCL6 and immunoglobulin family genes). The system was evaluated on 252 aggressive B-cell lymphomas from Europe and from sub-Saharan Africa. Using the algorithm, we determined a specific diagnosis of BL or not-BL in 82%, 92% and 95% cases at Phases 1, 2 and 3, respectively. In 3·4% cases, the algorithm was not completely applicable due to technical reasons. Overall, this approach led to a specific diagnosis of BL in 122 cases and to a specific diagnosis of either DLBCL or DLBCL/BL in 94% of cases that were not diagnosed as BL. We also evaluated the scoring system on 27 cases of BL confirmed on gene expression/microRNA expression profiling. Phase 1 of our scoring system led to a diagnosis of BL in 100% of these cases
URI
http://www.ncbi.nlm.nih.gov/pubmed/21718280http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/48289
Citation
Br J Haematol. 2011 Jul 1Publisher
Department of Pathology, University of Nairobi Department of Human Pathology and Oncology, University of Siena, Siena, Italy
Collections
- Faculty of Health Sciences (FHS) [10378]