dc.contributor.author | Naresh KN. | |
dc.contributor.author | Ibrahim HA. | |
dc.contributor.author | Lazzi S. | |
dc.contributor.author | Rince P. | |
dc.contributor.author | Onorati M. | |
dc.contributor.author | Ambrosio MR. | |
dc.contributor.author | Bilhou-Nabera C. | |
dc.contributor.author | Amen F. | |
dc.contributor.author | Reid A. | |
dc.contributor.author | Mawanda M. | |
dc.contributor.author | Calbi V. | |
dc.contributor.author | Ogwang M. | |
dc.contributor.author | Rogena Emily Adhiambo. | |
dc.contributor.author | Byakika B. | |
dc.contributor.author | Sayed S. | |
dc.contributor.author | Moshi E. | |
dc.contributor.author | Mwakigonja A. | |
dc.contributor.author | Raphael M. | |
dc.contributor.author | Magrath I. | |
dc.contributor.author | Leoncini L. | |
dc.date.accessioned | 2013-07-17T07:00:04Z | |
dc.date.available | 2013-07-17T07:00:04Z | |
dc.date.issued | 2011-07 | |
dc.identifier.citation | Br J Haematol. 2011 Jul 1 | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/21718280 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/48289 | |
dc.description.abstract | Distinguishing Burkitt lymphoma (BL) from B cell lymphoma, unclassifiable with features intermediate between diffuse large B-cell lymphoma (DLBCL) and BL (DLBCL/BL), and DLBCL is challenging. We propose an immunohistochemistry and fluorescent in situ hybridization (FISH) based scoring system that is employed in three phases - Phase 1 (morphology with CD10 and BCL2 immunostains), Phase 2 (CD38, CD44 and Ki-67 immunostains) and Phase 3 (FISH on paraffin sections for MYC, BCL2, BCL6 and immunoglobulin family genes). The system was evaluated on 252 aggressive B-cell lymphomas from Europe and from sub-Saharan Africa. Using the algorithm, we determined a specific diagnosis of BL or not-BL in 82%, 92% and 95% cases at Phases 1, 2 and 3, respectively. In 3·4% cases, the algorithm was not completely applicable due to technical reasons. Overall, this approach led to a specific diagnosis of BL in 122 cases and to a specific diagnosis of either DLBCL or DLBCL/BL in 94% of cases that were not diagnosed as BL. We also evaluated the scoring system on 27 cases of BL confirmed on gene expression/microRNA expression profiling. Phase 1 of our scoring system led to a diagnosis of BL in 100% of these cases | en |
dc.language.iso | en | en |
dc.subject | Lymphoma | en |
dc.subject | Diagnostic haematology | en |
dc.subject | Immunophenotyping | en |
dc.title | Diagnosis of Burkitt lymphoma using an algorithmic approach - applicable in both resource-poor and resource-rich countries. | en |
dc.type | Article | en |
local.publisher | Department of Pathology, University of Nairobi | en |
local.publisher | Department of Human Pathology and Oncology, University of Siena, Siena, Italy | en |