Mucosal and plasma IgA from HIV-1-exposed uninfected individuals inhibit HIV-1 523 transcytosis across human epithelial cells.
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Date
2000Author
Devito, C
Broliden, K
Kaul, R
Svensson, L
Johansen, K
Kiama, P
Kimani, JL
Lopalco, S
Bwayo, JJ
Clerici, M
Hinkula, J
Type
ArticleLanguage
enMetadata
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Abstract
HIV-1-specific IgA has been described in the genital tract and plasma of HIV-1 highly exposed, persistently seronegative (HEPS) individuals, and IgA from these sites has been shown to neutralize HIV-1. This study examines the ability of IgA isolated from HEPS individuals to inhibit transcytosis across a tight epithelial cell layer. A Transwell system was established to model HIV-1 infection across the human mucosal epithelium. The apical-basolateral transcytosis of primary HIV-1 isolates across this mucosal model was examined in the presence and the absence of IgA isolated from the genital tract, saliva, and plasma of HEPS individuals enrolled in both a sex worker cohort in Nairobi, Kenya, and a discordant couple cohort in Italy. In the absence of IgA, HIV-1 primary isolates were actively transported across the epithelial membrane and were released on the opposite side of the barrier. These transcytosed HIV-1 particles retained their ability to infect human mononuclear cells. However, IgA purified from the mucosa and plasma of HEPS individuals was able to inhibit HIV-1 transcytosis. Inhibition was seen in three of six cervicovaginal fluid samples, five of 10 saliva samples, and three of six plasma samples against at least one of the two primary HIV-1 isolates tested. IgA from low risk, healthy control subjects had no inhibitory effect on HIV-1 transcytosis. The ability of mucosal and plasma IgA to inhibit HIV-1 transcytosis across the mucosal epithelium may represent an important mechanism for protection against the sexual acquisition of HIV-1 infection in HEPS individuals.
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http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/51728http://www.ncbi.nlm.nih.gov/pubmed/11046049
Citation
J Immunol, 165, 5170-5176, 2000Publisher
Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya;
Collections
- Faculty of Health Sciences (FHS) [10387]