Evaluation of ICT filariasis card test using whole capillary blood: comparison with Knott's concentration and counting chamber methods
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Date
2001-10Author
Njenga, Sammy M
Wamae, C Njeri
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
An immunochromatographic card test (ICT) that uses fingerprick whole blood instead of serum for diagnosis of bancroftian filariasis has recently been developed. The card test was validated in the field in Kenya by comparing its sensitivity to the combined sensitivity of Knott's concentration and counting chamber methods. A total of 102 (14.6%) and 117 (16.7%) persons was found to be microfilaremic by Knott's concentration and counting chamber methods, respectively. The geometric mean intensities (GMI) were 74.6 microfilariae (mf)/ml and 256.5 mf/ml by Knott's concentration and counting chamber methods, respectively. All infected individuals detected by both Knott's concentration and counting chamber methods were also antigen positive by the ICT filariasis card test (100% sensitivity). Further, of 97 parasitologically amicrofilaremic persons, 24 (24.7%) were antigen positive by the ICT. The overall prevalence of antigenemia was 37.3%. Of 100 nonendemic area control persons, none was found to be filarial antigen positive (100% specificity). The results show that the new version of the ICT filariasis card test is a simple, sensitive, specific, and rapid test that is convenient in field settings.
URI
http://www.journalofparasitology.org/doi/abs/10.1645/0022-3395%282001%29087%5B1140%3AEOIFCT%5D2.0.CO%3B2?journalCode=parahttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/53959
Citation
Journal of Parasitology: October 2001, Vol. 87, No. 5, pp. 1140-1143.Publisher
University of Nairobi Faculty of medicine
Collections
- Faculty of Health Sciences (FHS) [10387]