Molecular Characterization of a Novel Plasmid-Encoded Cefotaximase (CTX-M-12) Found in Clinical Klebsiella pneumoniae Isolates from Kenya
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Date
2001Author
Kariuki, S
Corkill, JE
Revathi, G
Musoke, R
Hart, CA
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Nine Klebsiella pneumoniae isolates, six from blood and three from cerebrospinal fluid of newborn babies at
Kenyatta National Hospital, Nairobi, Kenya, were analyzed for the mechanism of cephalosporin resistance. By
using pulsed-field gel electrophoresis of XbaI-digested chromosomal DNA, all the nine isolates were found to
be clonal. PCR and direct sequencing revealed a novel extended-spectrum b-lactamase, which we designated
CTX-M-12. It has a more potent hydrolytic activity against cefotaxime than against ceftazidime and a pI of 9.0
and is encoded on a large self-transferable ca. 160-kbp plasmid
URI
http://aac.asm.org/content/45/7/2141.full.pdf+htmlhttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/54061
Citation
Kariuki, S., Corkill, J. E., Revathi, G., Musoke, R., & Hart, C. A. (2001). Molecular characterization of a novel plasmid-encoded cefotaximase (CTX-M-12) found in clinical Klebsiella pneumoniae isolates from Kenya. Antimicrobial agents and chemotherapy, 45(7), 2141-2143.Publisher
University of Nairobi, Department of Paediatric & Microbiology
Collections
- Faculty of Health Sciences (FHS) [10387]