dc.contributor.author | Ting, J | |
dc.contributor.author | Mugo, N | |
dc.contributor.author | Kwatampora, J | |
dc.contributor.author | Hill, C | |
dc.contributor.author | Chitwa, M | |
dc.contributor.author | Patel, S | |
dc.contributor.author | Gakure, H | |
dc.contributor.author | Kimani, J | |
dc.contributor.author | Schoenbach, VJ | |
dc.contributor.author | Poole, C | |
dc.contributor.author | Smith, JS | |
dc.date.accessioned | 2013-10-09T11:39:56Z | |
dc.date.available | 2013-10-09T11:39:56Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Sex Transm Dis. 2013 Jul;40(7):584-589. | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/23965776 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/57517 | |
dc.description.abstract | BACKGROUND:
Little is known about the performance of physician-versus self-collected specimens for high-risk human papillomavirus (hrHPV) messenger RNA (mRNA) testing or risk factors for hrHPV mRNA positivity in physician- versus self-collected specimens. We compared the performance of hrHPV mRNA testing of physician- and self-collected specimens for detecting cytological high-grade squamous intraepithelial lesions or more severe (≥HSIL) and examined risk factors for hrHPV mRNA positivity in female sex workers in Nairobi.
METHODS:
From 2009 to 2011, 344 female sex workers participated in this cross-sectional study. Women self-collected a cervicovaginal specimen. A physician conducted a pelvic examination to obtain a cervical specimen. Physician- and self-collected specimens were tested for hrHPV mRNA and sexually transmitted infections using APTIMA nucleic acid amplification assays (Hologic/Gen-Probe Incorporated, San Diego, CA). Cervical cytology was conducted using physician-collected specimens and classified according to the Bethesda criteria.
RESULTS:
Overall hrHPV mRNA prevalence was similar in physician- and self-collected specimens (30% vs. 29%). Prevalence of ≥HSIL was 4% (n = 15). Overall sensitivity of hrHPV testing for detecting ≥HSIL was similar in physician-collected (86%; 95% CI, 62%-98%; 13 cases detected) and self-collected specimens (79%; 95% CI, 55%-95%; 12 cases detected). Overall specificity of hrHPV mRNA for ≥HSIL was similar in both physician-collected (73%; 95% CI, 68%-79%) and self-collected (75%; 95% CI, 70%-79%) specimens. High-risk HPV mRNA positivity in both physician- and self-collected specimens seemed higher in women who were younger (<30 years), had Trichomonas vaginalis or Mycoplasma genitalium infections, or had more than 8 years of educational attainment.
CONCLUSIONS:
Self-collected specimens for hrHPV mRNA testing seemed to have similar sensitivity and specificity as physician-collected specimens for the detection of ≥HSIL among high-risk women. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | High-Risk Human Papillomavirus Messenger RNA Testing in Physician- and Self-Collected Specimens for Cervical Lesion Detection in High-Risk Women, Kenya. | en |
dc.type | Article | en |
local.publisher | Department of Obstetrics, | en |