Isolation and biological characterization of non-B HIV type 1 from Kenya
Date
2012-07Author
Muriuki, JK
Ngeranwa, JN
Mwangi, J
Orinda, G
Lwembe, R
Khamadi, S
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The isolation and characterization of primary strains of human immunodeficiency virus (HIV) is a vital tool for assessing properties of viruses replicating in HIV-infected subjects. HIV-1 isolation was carried out from 30 HIV-1-infected patients from a Comprehensive Care Clinic (CCC) after informed consent. Virus was successfully isolated from 9 out of the 30 samples investigated. Seven of the isolates were from drug-naive patients while two were from patients on antiretroviral drugs. The isolates were biologically phenotyped through measurement of the syncytium-inducing capacity in MT2 cells. Six of the isolates exhibited syncytia induction (SI) associated with CXCR4 coreceptor usage while three of the isolates were non-syncytia-inducing (NSI) isolates associated with CCR5 coreceptor usage. In addition, the replication capacity of the isolates was further determined in established cell line CD4(+) C8166. Indirect immunofluorescence assay was used to check the antigen expression on the cells as a supplementary test. HIV-1 isolation success was 70% (7/10) and 20% (2/20) in naive and drug-experienced patients, respectively. The majority of the viral isolates obtained (6/9) were of the SI phenotype, though SI virus strains are rare among non-B subtypes. A significant correlation between virus isolation success and viral load was established. Coreceptor use data for heavily treatment-experienced patients with limited treatment options are scanty and this is the group with perhaps the most urgent need of novel antiretroviral agents.
URI
http://www.ncbi.nlm.nih.gov/pubmed/22077875http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/57724
Citation
AIDS Res Hum Retroviruses. 2012 Jul;28(7):660-6. doi: 10.1089/AID.2010.0095. Epub 2012 Mar 12.Sponsorhip
Centre of Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.Publisher
School of Medicine, University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10387]