Liquid chromatographic separation of hexopyranosylated cytosine nucleosides from their degradation products
dc.contributor.author | Thoithi, G | |
dc.contributor.author | Van, Schepdael A | |
dc.contributor.author | Herdewijn, P | |
dc.contributor.author | Roets, E | |
dc.contributor.author | Hoogmartens, J | |
dc.date.accessioned | 2013-10-22T05:50:22Z | |
dc.date.available | 2013-10-22T05:50:22Z | |
dc.date.issued | 1997-11 | |
dc.identifier.citation | J Pharm Biomed Anal. 1997 Nov;16(3):533-40 | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/9589414 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/57773 | |
dc.description.abstract | Development of a liquid chromatographic method which can separate each of a series of hexopyranosylated cytosine nucleosides from their degradation products formed at acid, neutral and basic pH is described. Both silica-based reverse-phase and polymer columns were examined. Influence of the mobile phase pH, ion-pairing agent, concentration of the buffer and type and concentration of organic modifier were systematically investigated. The concentration of the ion-pairing agent and the buffer were found to have a major effect on selectivity. Samples were finally analyzed on a poly(styrene-divinylbenzene), PLRP-S 100 A (8 microns) 250 x 4.6 mm I.D. column at 60 degrees C and with a mobile phase consisting of acetonitrile-sodium octanesulphonate (pH 2.5; 0.02 M)-potassium phosphate buffer (pH 2.5; 0.2 M)-water (X:25:50:25-X, v/v, where X is variable). | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | Liquid chromatographic separation of hexopyranosylated cytosine nucleosides from their degradation products | en |
dc.type | Article | en |
local.publisher | school of public health | en |
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