Identification of three novel human leukocyte antigen alleles, HLA-B58:43 HLA-C03:190 and HLA-DPA101:12 in an East African cohort.
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Date
2013-08Author
Nykoluk, M
Bailey, RC
Moses, S
Plummer, FA
Luo, M.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Three novel human leukocyte antigen (HLA) alleles were identified using a sequence-based typing of HLA class I and class II alleles of 1867 participants from a male circumcision cohort in Kenya. The new alleles were first identified by sequencing and then confirmed by cloning the polymerase chain reaction (PCR) products and sequencing multiple clones. HLA-B*58:43 was identical to HLA-B*58:02 with the exception of a nucleotide change at codon 125 in exon 3 (GCC→ACC), and resulted in the amino acid change from Alanine to Threonine. HLA-C*03:190 was identical to HLA-C*03:02:01 with the exception of a nucleotide change at codon 131 in exon 3 (CGC→TGC), and resulted in the amino acid change from Arginine to Cysteine. HLA-DPA1*01:12 was identical to HLA-DPA1*01:03:01:01 with the exception of a nucleotide change at codon 66 in exon 2 (TTG→TCG), and resulted in the amino acid change from Leucine to Serine.
URI
http://www.ncbi.nlm.nih.gov/pubmed/23849069http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/60534
Citation
Tissue Antigens. 2013 Aug;82(2):131-3. doi: 10.1111/tan.12157Publisher
University of Nairobi HIV and Human Genetics, National Microbiology Laboratory
Collections
- Faculty of Health Sciences (FHS) [10387]