dc.contributor.author | Muzoora, Saphan | |
dc.date.accessioned | 2014-07-22T09:17:12Z | |
dc.date.available | 2014-07-22T09:17:12Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | University of Nairobi College of Agriculture and Veterinary Sciences Faculty of Veterinary Medicine 8th Biennial Scientific Conference and The 46th Kenya Veterinary Association Annual Scientific Conference and The 12th World Veterinary Day Celebrations | en_US |
dc.identifier.uri | http://hdl.handle.net/11295/73243 | |
dc.description.abstract | Background & Problem Statement: Recent studies have shown that certain strains of M. tuberculosis
are more virulent than others because these strains have been isolated from both immuno-competent
and immuno-compromised individuals. A recent study done in Uganda showed that Uganda strains
were the most prevalent. Genetic variations in these strains have been implicated as the cause
of variations in their virulence abilities. However, no definitive case links or specific genomic
characteristics regarding these strains have been detected which may have restricted efforts to reduce
their transmission.
Specific objectives: To define putative genes associated with virulence of Uganda strains and to
determine the association between virulence and specific genomic characteristics.
Methodology: The bacterial strains are archived isolates of five Uganda strains and three other East
African strains. H37Rv and H37Ra are being used as positive and negative controls respectively. The
DNA isolation is being done using a standard protocol. PCR is being used to amplify the DNA regions
of interest. Definition of putative genes and other genomic characteristics will be done using DNA
microarray analysis technique. Scanning of slides will be done in UK.
Ongoing activities: Extraction of DNA from the archived isolates and preparation of microarray
analysis slides.
Expected outcomes: Genomic characteristics responsible for the high virulence of Uganda strains
will be determined and the association between virulence and wide spread transmission established | en_US |
dc.title | Association between virulence and specific genomic characteristics of predominant Mycobacterium tuberculosis Uganda genotypes | en_US |
dc.type | Presentation | en_US |
dc.type.material | en | en_US |