dc.contributor.author | Amugune, B K | |
dc.contributor.author | Thoithi, G N | |
dc.contributor.author | Mwangi, JW | |
dc.contributor.author | Omosa, L K. | |
dc.contributor.author | Kibwage, IO | |
dc.date.accessioned | 2015-02-11T09:45:35Z | |
dc.date.available | 2015-02-11T09:45:35Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Amugune, B. K., Thoithi, G. N., Mwangi, J. W., Omosa, L. K., & Kibwage, I. O. (2015). Antimicrobial Activity and Bioactive Constituents of Alectra sessiliflora (Vahl) Kuntze Methanol Extract. East and Central African Journal of Pharmaceutical Sciences, 16(3), 61-68. | en_US |
dc.identifier.uri | http://hdl.handle.net/11295/80212 | |
dc.description.abstract | Alectra sessiliflora (Vahl) Kuntze (Scrophulariaceae) is traditionally used in western Kenya
in the management of microbial infections. The water, chloroform and methanol extracts of
A. sessiliflora whole plant exhibited antimicrobial activity against a range of bacteria
(Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Shigella dysenteriae and
Bacillus pumilus) and fungi (Candida albicans, Aspergillus niger and Cryptococcus
neoformans). The methanol extract exhibited the highest activity with minimum inhibitory
concentration (MIC) of 3.13-6.25 and 3.13-12.5 mg/ml for bacteria and fungi, respectively.
Chromatographic fractionation of the methanol extract through non-polar D101
macroporous resin beads yielded three bioactive compounds: two phenolic compounds, pcoumaric
acid and 3,4-dihydroxybenzoic acid, and a flavonoid, luteolin. The compounds
exhibited appreciable activities against tested bacteria and fungi with MIC values ranging
from 0.13 to 0.25 and 0.13 to 0.50 mg/ml, respectively. These constituents might be
responsible either individually or collectively for the traditional use of the plant to manage
bacterial and fungal ailments. The in vitro antimicrobial activity and isolation of bioactive
compounds from this plant are being reported for the first time. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.subject | Alectra sessiliflora, ethnomedicine, antibacterial and antifungal activity, bioactive constituents | en_US |
dc.title | Antimicrobial activity and bioactive constituents of alectra sessiliflora (vahl) kuntze methanol extract | en_US |
dc.type | Article | en_US |
dc.type.material | en_US | en_US |