Isolation of erythromycins and related substances from fermentation residues of strepto.'i1yces erythreus by high-performance liquid chromatography on silica gel
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Preparative high-performance liquid chromatography on silica gel allows the isolation of erythromycins and related substances from mother-liquor concentrates. Three mobile phases were used consecutively: A, ethyl acetate-methanol-25% am¬monia (100:8:1, v.v); B, diethyl ether-methanol-25% ammonia (l00:7:1. v,/v) and C, dichloromethane-methanol-25% ammonia (lOO:5:0,5, \i'v). The separation and puri¬fication was confirmed by thin-layer chromatography. Thirteen pure substances were isolated among which are erythromycins A, B, C and D, 8,9-anhydroerythromycin A-6,9-hemiketal, erythromycins A and C-6,9;6,12-spiroketals and N-demethylery¬thromycin A-6.9:9,12-spiroketal.