Characterisation of the vaginal microflora of human immunodeficiency virus (HIV) positive and negative women in a sub-urban population of Kenya.
Kiama, Teresa N.
Mbugua, Paul M.
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Lactobacilli predominate normal vaginal microflora and are important in maintenance of vaginal health. The current study set out to identify and compare c ulture isolates of vaginal microflora of human immunodeficiency virus ( HIV ) positive (HIV + ) and HIV negative (HIV - ) women at different p hase s during menstrual cycle from a sub - urban population of Kenya. Seventy four (74) women, 41 HIV + and 33 HIV - , followed up two consecutive menstrual cycles, had high vaginal swabs taken to prepare Gram s tains for six visits and anaerobic cultures for four. All 751 i solates identified by t - DNA polymerase chain reaction ( PCR ) belong to 51 species. Species cultured more frequently in HIV + p articipants were: Lactobacillus jensenii (p=0.01) , Lactobacillus iner s (p=0.02) , Gardnerella vaginalis (p=0.01) and Peptoniphilus lacrimalis (p=0.01). S pecies cultured more frequently in HIV - women were Dialister micraerophilus (p=0.02) and Streptococcus agalactiae (p=0.04) . Lactobacillus predominating both groups were Lact obacilli crispatus, L. jensenii, L. iners and Lactobacilli vaginalis . Bacterial vaginosis (BV) was equally high in HIV + and HIV - women. Lactobacillus and BV - associated species were cultured more frequently in HIV + women. Minor species differences were foun d. P redominant Lactobacillus in culture were L. crispatus , L. iners , L. jensenii and L. vaginalis . T hese women had lower concentrations of lactobacilli in vaginal microflora than observed in previous studies of Caucasian women