Elevation of Heterophilic antibodies to rabbit erythrocytes in human pathologic sera: quantitative studies by Elisa using a Glycosphingolipid antigen

Abstract

Human heterophilic hemagglutinin to rabbit erythrocytes was quantitatively determined by the hemagglutination test and enzyme linked immunosorbent assay (ELISA) using rabbit erythrocyte pentaglycosyl ceramide (CPH), Gal (a 1-3) Gal (fl 1-4) GlcNAc (fl 1-3) Gal (fl 1-4) Glc-Cer as the antigen. The antibody levels in sera from 74 Hanganutziu-Deicher (H-D) antibody-positive patients were significantly higher than those in sera from 55 healthy donors, and the correlation between the antibody levels detected by ELISA and hemagglutinin titers was significant. The antibody levels detected by ELISA correlated to the IgG antibody levels, while the hemagglutinin titers correlated to the IgM antibody levels. These results suggested that IgG levels as well as IgM levels against rabbit erythrocytes were elevated in H-D antibody-positive patients with various tumors and infectious diseases. In 4 out of 67 cancer patients collected randomly, abnormally high levels of anti-CPR IgG antibody were detected.