Susceptibility of locally cultivated groundnut (Arachis hypogaea) varieties to aflatoxin accumulation in Homa Bay County, Kenya
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Date
2017Author
Ndisio, Boaz
Wachira, Peter
Kagot, Victor
Okoth, Sheila
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Groundnut is one of the staple foods in many parts of the world. Due to its high nutrient content, the
nuts are liable to colonization by aflatoxigenic fungi and subsequent aflatoxin accumulation. This study
was aimed at determining susceptibility of locally grown groundnut varieties to Aspergillus flavus in
Homa Bay County, Western Kenya. A pretested questionnaire was used to survey agronomic practices
on groundnut cultivation in 75 randomly selected households in the study site. From each household
farm, 100 g soil samples and 500 g of groundnuts were collected at harvest and A. flavus isolated on
Modified Rose-Bengal Agar and identified. Aflatoxin was then extracted from each of the groundnut
samples and quantified using direct competitive enzyme linked immunosorbent assay (ELISA). Red
Valencia was the most cultivated among the 8 varieties identified. Farmers (66%) obtained the planting
seeds from the local market and most (92%) did not use fertilizers with majority (94%) having no
knowledge of aflatoxins. There was no significant inter-variety difference in aflatoxin accumulation
(p=0.744, F=0.581, Df=6, 61). A highly significant association (t = 2.652; P = 0.010) was found between
storage state of the groundnuts and aflatoxin levels, with 94% of the samples stored unshelled having
aflatoxin levels below 10 ppb. Overall, only 6.7% of kernels sampled from all the divisions did not meet
the EC aflatoxin limit of ≤4 ppb while 4% did not meet the KEBS limit of ≤10 ppb. Though the agronomic
practices were poor, aflatoxin levels were predominantly low in the region suggesting that the aflatoxin
accumulation is likely influenced by agro-ecological zoning as other studies have also been indicated.
URI
https://www.researchgate.net/publication/319957275_Susceptibility_of_locally_cultivated_groundnut_Arachis_hypogaea_varieties_to_aflatoxin_accumulation_in_Homa_Bay_County_Kenyahttp://hdl.handle.net/11295/101508
Citation
Boaz, Ndisio, et al. "Susceptibility of locally cultivated groundnut (Arachis hypogaea) varieties to aflatoxin accumulation in Homa Bay County, Kenya." African Journal of Microbiology Research 11.33 (2017): 1329-1337.Publisher
University of Nairobi
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Attribution-NonCommercial-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-nd/3.0/us/Collections
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