Ion-pair and Ion-suppression High Performance Liquid Chromatographic Analysis of Alkaloidal Constituents of Artabotrys monteiroae
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Date
1993Author
Kato, Atsushi
Moriyasu, Masataka
Nishiyama, Yumi
Ichimaru, Momoyo
Juma, Francis D.
Ogeto, John O.
Language
enMetadata
Show full item recordAbstract
lon-pair and ion-suppression mode high performance liquid chromatography was conducted on the alkaloidal constituents of the rools of Artabotrys monteiroae, an lmportant medicinal plant in Kenya having analgesic and anti-inflammatory action. Use of sodium perchlorate as an ion-pair reagent led to excellent separation. In the ion¬suppression system containing ammonium acetate, the separation was rather poor, but liquid chromatography ¬mass spectrometry was possible, which was not the case in the ion-pair system. Four aporphines, (-) ¬asimilobine, ( + )-wilsonirine, (+ )-norisoboldine and anonaine, and a benzylisoquinoline, (+ )-reticuline, were identilied alter isolation by preparative thin layer chromatography.
Publisher
Kobe Women's College of Pharmacy Department of Medicine. University of Nairobi Department of Pharmacy. University of Nairobi,
Subject
High performance liquid chromatography;Liquid chromatography-mass spectrometry;
Aporphine alkaloids
Benzylisoquinoline alkaloid
Artabotrys monteiroae