Ion-pair and Ion-suppression High Performance Liquid Chromatographic Analysis of Alkaloidal Constituents of Artabotrys monteiroae
Juma, Francis D.
Ogeto, John O.
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lon-pair and ion-suppression mode high performance liquid chromatography was conducted on the alkaloidal constituents of the rools of Artabotrys monteiroae, an lmportant medicinal plant in Kenya having analgesic and anti-inflammatory action. Use of sodium perchlorate as an ion-pair reagent led to excellent separation. In the ion¬suppression system containing ammonium acetate, the separation was rather poor, but liquid chromatography ¬mass spectrometry was possible, which was not the case in the ion-pair system. Four aporphines, (-) ¬asimilobine, ( + )-wilsonirine, (+ )-norisoboldine and anonaine, and a benzylisoquinoline, (+ )-reticuline, were identilied alter isolation by preparative thin layer chromatography.
Kobe Women's College of PharmacyDepartment of Medicine. University of NairobiDepartment of Pharmacy. University of Nairobi,
SubjectHigh performance liquid chromatography;
Liquid chromatography-mass spectrometry;