dc.contributor.author | Kato, Atsushi | |
dc.contributor.author | Moriyasu, Masataka | |
dc.contributor.author | Ichimaru, Momoyo | |
dc.contributor.author | Nishiyama, Yumi | |
dc.contributor.author | Juma, Francis D. | |
dc.contributor.author | Nganga, Joseph N | |
dc.contributor.author | Mathenge, Simon G. | |
dc.contributor.author | Ogeto, John O. | |
dc.date.accessioned | 2013-02-21T11:26:21Z | |
dc.date.issued | 1995 | |
dc.identifier.citation | Phytochemical analysis Vol. 6 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10609 | |
dc.description.abstract | Quaternary alkaloids of the stem of Zanthoxylum usambarense, an important medicinal plant in Kenya utilized for the treatment of pneumonia and rheumatism, were examined by a combination of ion-pair extraction and preparative ion-pair high performance liquid chromatography (HPLC), in which sodium perchlorate was used as an ion-pair reagent. Quaternary bases were extractable from aqueous solution into I ,2-dichloroethane in the presence of perchlorate in acidic conditions. The phenomenon is explained in terms of ion-pair extraction between alkaloidal cation and perchlorate anion. The quaternary alkaloidal fraction thus obtained was well separated by means of ion-pair HPLC in which sodium perchlorate was also utilized. Furthermore, the solvent system was easily applied to preparative HPL " yielding the pure perchlorate of each alkaloid. Eight quaternary bases in Z. usambarense were thus obtained and their structures were elucidated. One of them wa a new quaternary base of the tetrahydroproloberberine type, which was named (-)-usambarine. | en |
dc.language.iso | en | en |
dc.subject | Ion-pair extraction; | en |
dc.subject | ion-pair high performance liquid chromatography; | en |
dc.subject | sodium perchlorate; | en |
dc.subject | Quaternary alkaloids; | en |
dc.subject | (- )-usambarine; | en |
dc.subject | Zanthoxylum usambarense . | en |
dc.title | Examination of alkaloidal constituents of zanthoxylum usambarense by a combination of ion-pair extraction and ion-pair chromatography using sodium perchlorate | en |
dc.type | Article | en |
local.publisher | Department of Natural Medicinal Chemistry, Kobe Pharmaceutical University | en |
local.publisher | Department of Medicine, University of Nairobi | en |
local.publisher | Department of Botany, University of Nairobi | en |
local.publisher | Department of Pharmacy, University of Nairobi | en |