dc.contributor.author | Kiplagat, Chebon S | |
dc.contributor.author | Onyari, John M | |
dc.contributor.author | Mulaa, Francis | |
dc.contributor.author | Wabomba, John | |
dc.date.accessioned | 2019-09-11T07:29:24Z | |
dc.date.available | 2019-09-11T07:29:24Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | KIPLAGAT, CHEBON SAMMY, et al. "Extraction and characterization of gelatin from Lates niloticus and potential industrial applications." Biofarmasi Journal of Natural Product Biochemistry 15.2 (2017): 53-64. | en_US |
dc.identifier.uri | https://www.researchgate.net/publication/326361807_Extraction_and_characterization_of_gelatin_from_Lates_niloticus_and_potential_industrial_applications | |
dc.identifier.uri | http://erepository.uonbi.ac.ke/handle/11295/107104 | |
dc.description.abstract | Kiplagat CS, Onyari JM, Mulaa F, Wabomba J. 2018. Extraction and characterization of gelatin from Lates niloticus and
potential industrial applications. Biofarmasi J Nat Prod Biochem 16: 53-64. This research aims to extract and characterize gelatin from
Lates niloticus (Nile perch) scales, then blend it with polyvinyl alcohol (PVA). Hydrolysis of the scales was done using a crude
alkaline protease harvested from a bacterium, Bacillus cereus strain wwcp 1, obtained from Lake Bogoria. The lyophilized solution
yielded 16.3% of gelatin powder calculated from the dry weight of the scales. The sample was characterized using infrared spectroscopy
and showed peaks at 3442 cm-1
, 1653 cm-1
and ~ 1590 cm-1
corresponding to Amide A, Amide I and Amide II bands respectively. The
amino acid analysis shows that glycine was the most abundant amino acid (21.7%), followed by proline (14.6%) and alanine (11.8%).
Isoleucine, Histidine, and Tyrosine were the least abundant (1.8, 1.4 and 0.9% respectively). Polyvinyl alcohol-gelatin blend films of
various compositions ranging from 10% to 90% PVA were prepared by solution casting method. Differential Scanning Calorimetry
(DSC) and Thermo-gravimetric Analysis (TGA) tests showed the films had glass transition, melting and thermal decomposition onset
temperatures intermediate between those of the respective individual polymers ( PVA and gelatin). The thermal stability of the films
reduced with the increase in the amount of the less thermally stable constituent. Lastly, potential applications of the prepared blend films
were investigated. Batch experiments to assess the potential of the polymer blend films as an adsorbent material was done using
Methylene Blue dye. The films were found to adsorb up to 64% of the dye and the percentage of dye removal varied with the initial
concentration of the dye and contact time. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.subject | Gelatin, industrial applications, Lates niloticus | en_US |
dc.title | Extraction and characterization of gelatin from Lates niloticus and potential industrial applications | en_US |
dc.type | Article | en_US |