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    Evaluation of PetrifilmsTM as a diagnostic test to detect bovine mastitis organisms in Kenya

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    Date
    2012
    Author
    George, K. Gitau
    Royford, M. Bundi
    John, Vanleeuwen
    Charles, M. Mulei
    Type
    Article
    Language
    en
    Metadata
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    Abstract
    The study purpose was to validate PetrifilmsTM (3M Microbiology, 2005) against standard culture methods in the diagnosis of bovine mastitis organisms in Kenya. On 128 smallholder dairy cattle farms in Kenya, between June 21, 2010 and August 31, 2010, milk samples from 269 cows that were positive on California Mastitis Test (CMT) were cultured using standard laboratory culture methods and PetrifilmsTM (Aerobic Count and Coliform Count –3M Microbiology, 2005), and results were compared. Staphylococcus aureus was the most common bacterium isolated (73 % of samples). Clinical mastitis was found in only three cows, and there were only two Gram-negative isolates, making it impossible to examine the agreement between the two tests for Gram-negativeor clinical mastitis samples. The observed agreement between the standard culture and PetrifilmTM (3M Microbiology, 2005) results for Gram-positive isolates was 85 %, and there was fair agreement beyond that expected due to chance alone, with a kappa (κ) of 0.38. Using culture results as a gold standard, the PetrifilmsTM had a sensitivity of 90 % for Gram-positive samples and specificity of 51 %. With 87 % of CMT-positive samples resulting in Gram-positive pathogens cultured, there was a positive predictive value of 93 % and a negative predictive value of 43 %. PetrifilmsTM should be considered for culture of mastitis organisms in developing countries, especially when Gram-positive bacteria are expected.
    URI
    http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10952
    Citation
    Trop Anim Health Prod
    Publisher
    Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi
     
    Centre for Veterinary Epidemiologic Research, Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island
     
    Subject
    Dairy cattle
    Mastitis
    Laboratory culture
    PetrifilmsTM
    Test evaluation
    Kenya
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    • College of Agriculture and Veterinary Sciences (CAVS) [5286]

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