dc.contributor.author | Terkawi, M A | |
dc.contributor.author | Jia, H J | |
dc.contributor.author | Goo, Y-K | |
dc.contributor.author | Ooka, H | |
dc.contributor.author | Yamagishi, J | |
dc.contributor.author | Nishikawa, Y. | |
dc.contributor.author | Igarashi, I | |
dc.contributor.author | Fujisaki, K | |
dc.contributor.author | Xuan, X | |
dc.date.accessioned | 2013-02-26T12:08:17Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Parasite Immunology, 2009,31, 328–340 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/11812 | |
dc.description.abstract | Serological immunoscreening was used to identify a gene
encoding heat shock protein-70 from Babesia gibsoni
(BgHSP-70) that showed high homology with HSP-70s
from other apicomplexan parasites. This gene corresponded
to a full-length cDNA containing an open reading frame of
1968 bp predicted to result in a 70-kDa mature protein consisting
of 656 amino acids. Analysis of the expression levels
of BgHSP-70 indicated elevated transcription from cultured
parasites incubated at 40C for 1 h, but not at 30C. Interestingly,
antiserum raised against recombinant BgHSP-70
protein reacted specifically not only with a 70-kDa protein
of B. gibsoni but also with a corresponding native protein of
B. microti (BmHSP-70), indicating the high degree of conservation
of this protein. The BmHSP-70 gene was then isolated
and characterized and the immunoprotective properties
of recombinant BgHSP-70 (rBgHSP-70) and rBmHSP-70
were compared in vitro and in vivo. Both proteins had potent
mitogenic effects on murine and canine mononuclear cells as
evidenced by high proliferative responses and IFN-c production
after stimulation. Immunization regimes in BALB⁄c
and C57BL⁄6 mice using rBgHSP-70 and rBmHSP-70 elicited
high antibody levels, with concurrent significant reductions
in peripheral parasitaemias. Taken together, these
results emphasize the potential of HSP-70s as a molecular
adjuvant vaccine. | en |
dc.language.iso | en | en |
dc.subject | Babesia gibsoni | en |
dc.subject | Babesia microti | en |
dc.subject | Heat shock | en |
dc.subject | Protein-70 | en |
dc.title | Molecularand immunological characterization of Babesia gibsoni and Babesia microti heat shockprotein-70 | en |
dc.type | Article | en |
local.publisher | Department of Public Health Pharmacology and Toxicology | en |