dc.contributor.author | Jackson N, Ombui | |
dc.contributor.author | Horst, Schmieger | |
dc.contributor.author | Michael M, Kagiko | |
dc.contributor.author | Sammel, M | |
dc.date.accessioned | 2013-02-28T07:00:58Z | |
dc.date.issued | 1997 | |
dc.identifier.citation | FEMS Microbiology Letters 149 (1997) 245-248 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/12219 | |
dc.description.abstract | Bacillus cereus strains were tested for production of diarrheal enterotoxin by the reverse passive latex agglutination test and for presence of B. cereus enterotoxin gene (beeT) by polymerase chain reaction. About 50% of 56 B. cereus strains reacted positive in broth culture in the reverse passive latex agglutination test, while the beeT gene was detected in 41.1 %. Sixteen percent of the strains were positive for both diarrheal enterotoxin and bceT gene. A 741 bp probe prepared from the polymerase chain reaction product detected beeT gene in all strains that were positive with the polymerase chain reaction. This study indicates a likelihood of two or more enterotoxins being produced by B. cereus which may be involved in causing diarrheal type food poisoning. | en |
dc.language.iso | en | en |
dc.subject | Bacillus cereus | en |
dc.subject | Polymerase chain reaction | en |
dc.subject | Hybridization | en |
dc.subject | Enterotoxin | en |
dc.title | Bacillus cereus may produce two or more diarrheal enterotoxins | en |
dc.type | Article | en |
local.publisher | Department of Public Health, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Nairobi | en |
local.publisher | Institut fur Genetik und Mikrobiologie, Universitdt Miinchen. Maria Ward Str. l a, D-80638 Munich, Germany | en |